twa1 antibody (Thermo Fisher)
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Twa1 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/twa1+antibody/thermo+with+aba+response+1/10__7554_slash_elife__77937-372-43-44
Average 90 stars, based on 1 article reviews
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Western Blot:Article Title: GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme Article Snippet: The obtained lysate was cleared by centrifugation at 23,000 x g for 30 minutes at 4 C. 3mg of total protein were loaded on top of a 5%–40% sucrose gradient (weight/volume, in lysis buffer) and centrifuged in a SW60 rotor at 34,300 rpm for 16 hours at 4 C. Fractions were collected from top of the Article Title: Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation Article Snippet: For immunoblot analysis, lysates were denatured with SDS sample buffer, boiled at 95°C for 5 min, separated on SDS- PAGE, and proteins were visualized by immunoblotting using indicated primary antibodies: RMND5A (Santa Cruz), SDS Page:Article Title: GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme Article Snippet: The obtained lysate was cleared by centrifugation at 23,000 x g for 30 minutes at 4 C. 3mg of total protein were loaded on top of a 5%–40% sucrose gradient (weight/volume, in lysis buffer) and centrifuged in a SW60 rotor at 34,300 rpm for 16 hours at 4 C. Fractions were collected from top of the Article Title: Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation Article Snippet: For immunoblot analysis, lysates were denatured with SDS sample buffer, boiled at 95°C for 5 min, separated on SDS- PAGE, and proteins were visualized by immunoblotting using indicated primary antibodies: RMND5A (Santa Cruz), Centrifugation:Article Title: GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme Article Snippet: The obtained lysate was cleared by centrifugation at 23,000 x g for 30 minutes at 4 C. 3mg of total protein were loaded on top of a 5%–40% sucrose gradient (weight/volume, in lysis buffer) and centrifuged in a SW60 rotor at 34,300 rpm for 16 hours at 4 C. Fractions were collected from top of the Article Title: Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation Article Snippet: For immunoblot analysis, lysates were denatured with SDS sample buffer, boiled at 95°C for 5 min, separated on SDS- PAGE, and proteins were visualized by immunoblotting using indicated primary antibodies: RMND5A (Santa Cruz), Lysis:Article Title: GID E3 ligase supramolecular chelate assembly configures multipronged ubiquitin targeting of an oligomeric metabolic enzyme Article Snippet: The obtained lysate was cleared by centrifugation at 23,000 x g for 30 minutes at 4 C. 3mg of total protein were loaded on top of a 5%–40% sucrose gradient (weight/volume, in lysis buffer) and centrifuged in a SW60 rotor at 34,300 rpm for 16 hours at 4 C. Fractions were collected from top of the Article Title: Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation Article Snippet: For immunoblot analysis, lysates were denatured with SDS sample buffer, boiled at 95°C for 5 min, separated on SDS- PAGE, and proteins were visualized by immunoblotting using indicated primary antibodies: RMND5A (Santa Cruz), |

